产品名称 FGF-basic antibody | anti FGF-basic antibody | Biorbyt
产品货号 orb303490
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name FGF-basic antibody
Catalog Number orb303490
Species/HostMouse
ReactivityHuman
ConjugationUnconjugated
Tested applicationsIHC-P, S-ELISA, WB
Immunogen Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic as the immunizing antigen
Target FGF-basic
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
ClonalityMonoclonal
Purity Protein A affinity chromatography.
Uniprot ID P09038 (Human)
Dilution Range WB: 0.25-0.50 μg/ml, IHC-P: 0.25-0.50 μg/ml
Application Notes WB Notes: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-basic is 1.0-2.0 ng/lane, under non-reducing conditions.
ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human FGF-basic when used with Biorbyt' biotinylated antigen affinity purified anti-human FGF-basic
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 0.25-0.50 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with DAB chromogen. Heat induced antigen retrieval was performed with a pH 6.0 Sodium Citrate buffer. Optimal concentrations and conditions may vary.
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