产品名称 IL-12 antibody | anti IL-12 antibody | Biorbyt
产品货号 orb303578
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name IL-12 antibody
Catalog Number orb303578
Species/HostMouse
ReactivityHuman
ConjugationUnconjugated
Tested applicationsIHC-P, S-ELISA, WB
Immunogen Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human IL-12 as the immunizing antigen
Target IL-12
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
ClonalityMonoclonal
Purity Protein A affinity chromatography.
Uniprot ID P29459 (Human)
Dilution Range WB: 0.50-1.0 μg/ml, IHC-P: 10.0 μg/ml
Application Notes WB Notes: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.50-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 0.5-1.0 ng/lane, under non-reducing conditions.
ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human IL-12 when used with Biorbyt' biotinylated antigen affinity purified anti-human IL-12
IHC Notes: This antibody stained PBMC.  The primary antibody was incubated at 10.0 µg/ml for one hour at room temperature followed by a fluorescent labeled secondary antibody.  Optimal concentrations and conditions may vary.
Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia. 

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