产品名称 MCP-1 antibody | anti MCP-1 antibody | Biorbyt
产品货号 orb303732
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name MCP-1 antibody
Catalog Number orb303732
Species/HostMouse
ReactivityHuman
ConjugationUnconjugated
Tested applicationsIHC-P, S-ELISA, WB
Immunogen Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human MCP-1/MCAF as the immunizing antigen
Target MCP-1
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
ClonalityMonoclonal
Purity Protein A affinity chromatography.
Uniprot ID P13500 (Human)
Dilution Range WB: 0.20-0.40 μg/ml, IHC-P: 10.0 μg/ml
Application Notes WB Notes: To detect hMCP-1 by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1 is 1.0-2.0 ng/lane, under reducing or non-reducing conditions.
ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 300 pg/ml of recombinant human MCP-1 when used with Biorbyt' biotinylated antigen affinity purified anti-human MCP-1
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 10.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
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