产品名称 TNF-alpha antibody (Biotin) | anti TNF-alpha antibody (Biotin) | Biorbyt
产品货号 orb303930
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name TNF-alpha antibody (Biotin)
Catalog Number orb303930
Species/Host Rabbit
Reactivity Rat
Conjugation Biotin
Tested applications S-ELISA, WB
Immunogen Produced from sera of rabbits pre-immunized with highly pure recombinant Rat TNF-alpha
Target TNF-alpha (Biotin)
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by affinity chromatography
Uniprot ID P16599 (Rat)
Dilution Range WB: 0.1-0.2 μg/ml
Application Notes WB Notes: To detect Rat TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Notes: To detect Rat TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required.

Conjugation Notes: To detect Rat TNF-α by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat TNF-α.
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