产品名称 beta-NGF antibody | anti beta-NGF antibody | Biorbyt
产品货号 orb303900
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name beta-NGF antibody
Catalog Number orb303900
Species/HostMouse
ReactivityHuman
ConjugationUnconjugated
Tested applicationsIHC-P, S-ELISA, WB
Immunogen Produced in mice using highly pure recombinant human beta-NGF as the immunizing antigen
Target beta-NGF
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
ClonalityMonoclonal
Purity Protein G affinity chromatography.
Uniprot ID P01138 (Human)
Dilution Range WB: 4.0-8.0 μg/ml, IHC-P: 2.5 μg/ml
Application Notes WB Notes: To detect Human β-NGF by Western Blot analysis this antibody can be used at a concentration of 4.0-8.0 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human β-NGF is 4.0-8.0 ng/lane, under reducing or non-reducing conditions.
ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 200 pg/ml of recombinant Human β-NGF when used with Biorbyt' biotinylated antigen affinity purified anti-Human β-NGF
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas adenocarcinoma.  The recommended concentration is 2.5 µg/mL with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 9.0 buffer is recommended.  See photo(s).  Optimal concentrations and conditions may vary.
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