产品名称 VEGF antibody | anti VEGF antibody | Biorbyt
产品货号 orb303953
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name VEGF antibody
Catalog Number orb303953
Species/Host Rabbit
Reactivity Mouse
Conjugation Unconjugated
Tested applications IHC-P, S-ELISA, WB
Immunogen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mVEGF
Target VEGF
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by affinity chromatography
Uniprot ID Q00731-2 (Mouse)
Dilution Range WB: 0.1-0.2 μg/ml, IHC-P: 2.5μg/ml
Application Notes WB Notes: To detect mVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Notes: To detect mVEGF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of normal murine kidney. The recommended concentration is 2.5μg/ml for one hour at room temperature. A secondary fluorophore conjugated antibody was used for thirty minutes at room temperature. High pH heat induced antigen retrieval is recommended. Optimal concentrations and conditions may vary.
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