| 产品名称 |
Human BMP2 protein | Biorbyt |
| 产品货号 |
orb80007 |
| 产品价格 |
现货询价，电话：010-67529703
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| 产品规格 |
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| 产品品牌 |
Biorbyt |
| 产品概述 |
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| 产品详情 |
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| Product Name | Human BMP2 protein |
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| Catalog Number | orb80007 |
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| Conjugation | Unconjugated |
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| Tested applications | SDS-PAGE |
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| Target | BMP2 |
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| Alternative Names | (click to expand) BMP-2 protein, BMP2A protein |
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| Form/Appearance | BMP-2 was lyophilized from concentrated (1mg/ml) sterile solution containing 20 mM NaAc pH=4. |
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| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
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| Note | For research use only. |
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| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His. |
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| Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
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| Biological Origin | E.coli |
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| Activity | 1. The ED50 as determined by the cytolysis of MC3T3-E1 cells is 50 ng/ml. 2. When implanted in the mouse thighbone muscle and grow for 14 days, Muscle tissue was harvested and examined biochemically for calcium uptake. Our unit defined as microgram calcium absorbed. The specific activity is more than 1000 Units/mg. 3. The activity of BMP-2 was tested using alkaline phosphatase, the activity in mouse muscle myoblast C2C12C ell line was found to be 1 100,000 U/mg protein. 4. The ectopic osteogenesis capacity is 1000mg fresh bone per 1mg of BMP-2. |
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| Application Notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of BMP-2 as Reference Standard. |
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| Solubility (25°C) | It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20 mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
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| 产品资料 |
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