产品名称 Goat Serum Against Human IgD
产品货号 orb21893
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Goat Serum Against Human IgD
Catalog Number orb21893
Species/HostGoat
ConjugationUnconjugated
Tested applicationsDOT, IEP
Immunogen Highly purified monoclonal IgD isolated from human serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target IgD. The reactivity of the antiserum is restricted to the Fc part of the IgD molecule. In immuno- electrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum containing monoclonal IgD a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgD. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments.
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
Preservatives No preservative added
Storage The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Note For research use only.
ClonalityPolyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Notes The antiserum does not cross-react with any other component of the human immuno- globulin system. Inter-species cross-reactivity is a normal feature of antibodies to immuno- globulins, since Ig of different species frequently share antigenic determinants. Cross- reactivity of this antiserum has not been tested in detail.

IE: In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ulantiserum in 3 mm diameter center well and 2 μl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
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