产品名称 NANOG antibody
产品货号 orb19063
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name NANOG antibody
Catalog Number orb19063
Species/HostGoat
ReactivityHuman
Predicted Reactivity Canine, Porcine
ConjugationUnconjugated
Tested applicationsICC, IF, IHC-P, P-ELISA, WB
Immunogen Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
Target NANOG
Alternative Names
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MW 34.6 kDa
Uniprot ID Q9H9S0
NCBI NP_079141.2
Entrez 79923
Dilution Range P-ELISA: 1:64000, WB: 0.03-0.1 μg/ml
Application Notes ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.

WB: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.1). Recommended concentration: 0.03-0.1 μg/ml.

Experiment Notes: Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). Immunofluorescence: see also publication Raya A. et al., Nature. 2009 Jul 2;460(7251):53-9. Epub 2009 May 31. (PMID: 19483674). This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
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