产品名称 IgG antibody | Biorbyt
产品货号 orb108246
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name IgG antibody
Catalog Number orb108246
Species/Host Goat
Conjugation Unconjugated
Tested applications ELISA, IHC-Fr, WB
Target IgG. Based on immunoelectrophoresis, the antibody reacts with both heavy and light chain of Human IgG andwith light chains common to most human immunoglobulins. No antibody was detected against nonimmunoglobulinserum proteins, but the antibody may cross react with immunoglobulins from other species.
Form/Appearance Lyophilized from 0.01M Sodium Phosphate, 0.25M Sodium Chloride, pH 7.6 containing 15mg/ml BSA (IgG-Free, Protease-Free)
Concentration 0.8mg/ml (prior to lyophilization)
Preservatives No Preservative.
Storage Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks at 2-8°C as an undiluted liquid. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol (ACS or better grade) to make a final glycerol concentration of 50% followed by storage at-20°C, with or without aliquoting. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Expiration date is one year from date of reconstitution.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Affinity purified
Biological Origin Sample obtained from Goat.
Dilution Range IHC-Fr:1:500-1:5,000, ELISA:1:5,000-1:100,000, WB:1:10,000-1:200,000
Application Notes A dilution range of 1:500-1:5,000 is suggested for immunohistochemistry on tissue sections, 1:5,000 -1:100,000 for ELISA and Western blotting with chromogenic substrates, and 1:10,000-1:200,000 forWestern blotting with ECL substrates.Each laboratory should determine an optimum working titer for use inits particular application.Other applications have not been tested but use in such assays should notnecessarily be excluded.Use of sodium azide as a preservative will substantially inhibit the enzyme activity ofhorseradish peroxidase.Centrifuge before opening to ensure complete recovery of vial contents.
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