产品名称 IgG antibody | Biorbyt
产品货号 orb108264
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name IgG antibody
Catalog Number orb108264
Species/Host Goat
Conjugation Unconjugated
Tested applications ELISA, IHC-Fr, WB
Target IgG. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on bovine IgG and with lightchains common to most bovine immunoglobulins. No antibody was detected against non immunoglobulinserum proteins, but antibodies may cross react with immunoglobulins from other species.
Form/Appearance Lyophilized from 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.6 containing 15 mg/mL BSA (IgG and Protease Free).
Concentration 2 mg/mL (prior to lyophilization)
Preservatives NaN3.
Storage Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks at 2-8°C as an undiluted liquid. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest adding an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at-20°C, with or without aliquoting. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. Expiration date is one year from date of reconstitution.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Affinity purified
Biological Origin Sample obtained from Goat.
Dilution Range ELISA:1:20,000-1:400,000, WB:1:500-1:5,000, IHC-Fr:1:200-1:1,000
Application Notes A dilution range of 1:20,000-1:400,000 is suggested for ELISA and Western blotting using enzymeconjugatedstreptavidin, 1:500-1:5,000 for enzyme immunohistochemistry on tissue sections and 1:200 -1:1,000 for flow cytometry and fluorescence on immunohisto/cytochemistry.Each laboratory shoulddetermine an optimum working titer for use in its particular application.Other applications have not beentested but use in such assays should not necessarily be excluded.
产品资料