产品名称 IgG antibody | Biorbyt
产品货号 orb108252
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name IgG antibody
Catalog Number orb108252
Species/Host Goat
Conjugation Unconjugated
Tested applications ELISA, IHC-Fr, WB
Target IgG. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rabbit IgG and with lightchains common to most rabbit immunoglobulins. No antibody was detected against non immunoglobulinserum proteins, but antibodies may cross react with immunoglobulins from other species.
Form/Appearance Lyophilized from 0.01M Sodium Phosphate, 0.25M Sodium chloride, pH 7.6 containing 15mg/ml BSA
Concentration 0.8 mg/ml (prior to lyophilization)
Preservatives No Preservative.
Storage Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks at 2-8°C as an undiluted liquid. Prepare working dilution only prior to immediate use. For extended storage after reconstitution, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50% followed by storage at-20°C, with or without aliquoting. Please note that the concentration of protein and buffer salts will decrease to one-half of the original after the addition of glycerol. In the absence of glycerol, aliquot and store at-70°C or below. Avoid multiple freeze/thaw cycles. Expiration date is one year from date of reconstitution.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Affinity purified
Biological Origin Sample obtained from Goat.
Dilution Range IHC-Fr:1:500-1:5,000, ELISA:1:5,000-1:100,000, WB:1:10,000-1:200,000
Application Notes A dilution range of 1:500-1:5,000 is suggested for immunohistochemistry on tissue sections, 1:5,000-1:100,000 for ELISA and Western blots with chromogenic substrates and 1:10,000-1:200,000 for Westernblotting with ECL substrates.Each laboratory should determine an optimum working titer for use in itsparticular application.Other applications have not been tested but use in such assays should not necessarilybe excluded.Use of sodium azide as a preservative will substantially inhibit the enzyme activity ofhorseradish peroxidase.Centrifuge before opening to ensure complete recovery of vial contents.
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