| Application Notes |
Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse CA4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum and plasma(heparin, EDTA, citrate), urine to each empty well. See "Sample Dilution Guideline" above for details. We recommended that each mouse CA4 standard solution and each sample be measured in duplicate. |