| Application Notes |
Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187pg/ml, 93.8pg/ml human CILP standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA or citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommended that each human CILP standard solution and each sample be measured in duplicate. |