产品名称 | Swine Serum Against Rabbit IgG |
产品货号 | orb22467 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | Swine Serum Against Rabbit IgG |
---|---|
Catalog Number | orb22467 |
Species/Host | Porcine |
Conjugation | Unconjugated |
Tested applications | DOT, IEP |
Immunogen | Purified normal IgG isolated from pooled rabbit serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
Target | IgG. The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in rabbit serum, as tested by immunoelectrophoresis and double radial immunodiffusion. |
Form/Appearance | Vial with 1 ml lyophilized antiserum. |
Concentration | IgG protein concentration 1 mg/ml. No foreign proteins added. |
Preservatives | No preservative added, as it may interfere with the antibody activity. No foreign protein added. |
Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
Note | For research use only. |
Clonality | Polyclonal |
Adsorption | No adsorption required. |
Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
产品资料 |