产品名称 Swine IgG Against Human Ig Kappa Light Chains
产品货号 orb21577
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Swine IgG Against Human Ig Kappa Light Chains
Catalog Number orb21577
Species/Host Porcine
Conjugation Unconjugated
Tested applications DOT, IEP
Immunogen A pool of purified Bence Jones kappa proteins isolated from human urine. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target Ig Kappa (light chain). The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma.
Form/Appearance Vial with 10 mg lyophilized IgG (7S) preparation.
Concentration IgG protein concentration is 1 mg/ml. No foreign proteins added.
Preservatives No preservative added, as it may interfere with the antibody activity
Storage The lyophilised product is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Note For research use only.
Clonality Polyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins.
Application Notes Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.

Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt- precipitation and purification of the IgG fraction by DEAE-chromatography.
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