| 产品名称 | Goat Serum Against Human IgG Fab |
| 产品货号 | orb21771 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Goat Serum Against Human IgG Fab |
|---|---|
| Catalog Number | orb21771 |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Tested applications | DOT, IEP |
| Immunogen | Purified Fab of normal human IgG. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | IgG Fab. The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab)2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. |
| Form/Appearance | Vial with 1 ml lyophilized antiserum. |
| Concentration | Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added. |
| Preservatives | 0.1% sodium azide. |
| Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with other subunits of the immunoglobulin or reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of foreign protein or immune complexes in the antiserum. |
| Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in double radial immunodiffusion with the following results: bovine - cat - chicken - dog - guinea pig - hamster - horse - monkey ++ mouse - pigeon - rabbit - rat - sheep - swine - turkey - Three monkey sera were tested. A reaction with Rhesus monkey, Cynomolgus and Baboon have been found. IE: In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ulantiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. |
| 产品资料 |