产品名称 C3C antibody (FITC) | anti C3C antibody (FITC) | Biorbyt
产品货号 orb22111
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name C3C antibody (FITC)
Catalog Number orb22111
Species/Host Goat
Reactivity Monkey
Conjugation FITC
Tested applications DOT, IEP
Target C3C (FITC). In immunoelectrophoresis against fresh monkey serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of monkey serum or plasma.
Form/Appearance Vial with 1 ml lyophilized immunoconjugate.
Concentration IgG protein concentration 1 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.9. No foreign proteins added.
Preservatives No preservative added, as it may interfere with the antibody activity.
Storage The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Pr ior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and prefera bly used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Note For research use only.
Clonality Polyclonal
Adsorption Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Notes The antiserum does not cross-react with any other component of monkey plasma. Inter-species cross- reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. In addition to a clear reactivity with C3c of other old-world monkeys (Cercopithecus, Cynomolgus and Baboon), this antiserum shows also a fair amount of cross-reactivity to C3C of other species, including chimpanzee and man.

Conjugation Notes: Fluorescein isothiocyanate isomer 1 (FITC). Excitation: 492 nm, emission: 515 nm.

Experiment Notes: The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion.
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