产品名称 Milk Specific Proteins antibody | anti Milk Specific Proteins antibody | Biorbyt
产品货号 orb22392
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Milk Specific Proteins antibody
Catalog Number orb22392
Species/Host Rabbit
Reactivity Mouse
Conjugation Unconjugated
Tested applications DOT, IEP
Immunogen Pooled mouse milk. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target Milk Specific Proteins. In immunoelectrophoresis against mouse milk precipitation of not less than 7 different proteins may be obtained. However the number of visible precipitin lines varies among individual specimens of mouse milk. The total concentration of protein, their proportion of the different protein components change markedly during the course of lactation. There are also strain differences in the protein composition, but differences among individual animals tend to be greater. A protein detected in a mouse secretion by this antiserum is of secretory origin. However an analogous or homologous protein may be present also n other kind of secretions (e.g. saliva). Not all precipitable proteins have been identified. Known proteins include free secretory component and lactoferrin.
Form/Appearance Vial with 1 ml lyophilized antiserum.
Concentration Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Preservatives No preservative added.
Storage The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day.
Note For research use only.
Clonality Polyclonal
Adsorption Immunoaffinity adsorbed with normal mouse serum fractions to eliminate antibodies reacting with mouse serum proteins.
Application Notes Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross reactivity. Consequently it is not species- specific.

IE: In immunoelectrophoresis use 2 μl milk or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl milk samples (neat and serially diluted in 2 mm diameter peripheral wells.
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