产品名称 | Milk Specific Proteins antibody | anti Milk Specific Proteins antibody | Biorbyt |
产品货号 | orb22392 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | Milk Specific Proteins antibody |
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Catalog Number | orb22392 |
Species/Host | Rabbit |
Reactivity | Mouse |
Conjugation | Unconjugated |
Tested applications | DOT, IEP |
Immunogen | Pooled mouse milk. Freunds complete adjuvant is used in the first step of the immunization procedure. |
Target | Milk Specific Proteins. In immunoelectrophoresis against mouse milk precipitation of not less than 7 different proteins may be obtained. However the number of visible precipitin lines varies among individual specimens of mouse milk. The total concentration of protein, their proportion of the different protein components change markedly during the course of lactation. There are also strain differences in the protein composition, but differences among individual animals tend to be greater. A protein detected in a mouse secretion by this antiserum is of secretory origin. However an analogous or homologous protein may be present also n other kind of secretions (e.g. saliva). Not all precipitable proteins have been identified. Known proteins include free secretory component and lactoferrin. |
Form/Appearance | Vial with 1 ml lyophilized antiserum. |
Concentration | Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. |
Preservatives | No preservative added. |
Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day. |
Note | For research use only. |
Clonality | Polyclonal |
Adsorption | Immunoaffinity adsorbed with normal mouse serum fractions to eliminate antibodies reacting with mouse serum proteins. |
Application Notes | Inter-species cross reactivity is a normal feature of antibodies to animal proteins since homologous proteins of different species frequently share antigenic determinants. This antiserum has not been adsorbed for such cross reactivity. Consequently it is not species- specific. IE: In immunoelectrophoresis use 2 μl milk or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl milk samples (neat and serially diluted in 2 mm diameter peripheral wells. |
产品资料 |