产品名称 Serum Proteins antibody (FITC) | anti Serum Proteins antibody (FITC) | Biorbyt
产品货号 orb21752
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Serum Proteins antibody (FITC)
Catalog Number orb21752
Species/Host Goat
Reactivity Rabbit
Conjugation FITC
Tested applications DOT, IEP
Immunogen Pooled whole serum and selected protein fractions isolated from pooled rabbit serum. Freunds complete adjuvant is used in the first step of the immunization procedure.
Target Serum Proteins (FITC). Tested in immunoelectrophoresis and double radial immunodiffusion against pooled serum precipitation of at least 14 individual proteins have been observed.
Form/Appearance Vial with 1 ml lyophilized immunoconjugate.
Concentration IgG protein concentration 1 mg/ml. Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.6. No foreign proteins added.
Preservatives No preservative added.
Storage The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C , not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Note For research use only.
Clonality Polyclonal
Adsorption No adsorption required.
Application Notes Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross- reactivity of this antiserum has not been tested in detail.

Conjugation Notes: A proprietary technique for the binding to FITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Fluorescein isothiocyanate isomer 1. Excitation: 492 nm, emission: 515 nm.

Experiment Notes: Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
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