| 产品名称 | Dnp-Albumin antibody | anti Dnp-Albumin antibody | Biorbyt |
| 产品货号 | orb22366 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Dnp-Albumin antibody |
|---|---|
| Catalog Number | orb22366 |
| Species/Host | Rabbit |
| Reactivity | Monkey |
| Conjugation | Unconjugated |
| Tested applications | DOT, IEP |
| Immunogen | Purified albumin isolated from pooled monkey serum, coupled to dinitrophenol. Freunds complete adjuvant is used in the first step of the immunization procedure. |
| Target | Dnp-Albumin. The reactivity of the antiserum is restricted to albumin and albuminDNP as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified albumin. |
| Form/Appearance | Vial with 1 ml lyophilized antiserum. |
| Concentration | Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. |
| Preservatives | No preservative added. |
| Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | Inter-species cross reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail, however a reaction has been observed with human albumin, chicken ovalbuminDNP, but not with native chicken ovalbumin. IE: In immunoelectrophoresis use 2 μl serum or equivalent or DNP-conjugates against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion (Mancini) use 0.5 to 1% antiserum in the agar gel. |
| 产品资料 |