产品名称 | Albumin antibody | anti Albumin antibody | Biorbyt |
产品货号 | orb22365 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | Albumin antibody |
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Catalog Number | orb22365 |
Species/Host | Rabbit |
Reactivity | Monkey |
Conjugation | Unconjugated |
Tested applications | DOT, IEP |
Immunogen | Highly purified albumin isolated from monkey serum. Freunds complete adjuvant is used in the first step of the immunization procedure. |
Target | Albumin. The defined antibody specificity is directed to albumin as tested against monkey sera. In immuno- electrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against monkey serum and the purified albumin. |
Form/Appearance | Vial with 1 ml lyophilized antiserum. |
Concentration | Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added. |
Preservatives | No preservative added. |
Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day. |
Note | For research use only. |
Clonality | Polyclonal |
Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has not been tested for cross-reactivity. IE: In immunoelectrophoresis use 2 μl serum, plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel. |
产品资料 |