| 产品名称 | C3C antibody | anti C3C antibody | Biorbyt |
| 产品货号 | orb21905 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | C3C antibody |
|---|---|
| Catalog Number | orb21905 |
| Species/Host | Goat |
| Reactivity | Guinea pig |
| Conjugation | Unconjugated |
| Tested applications | DOT, IEP |
| Target | C3C. In immunoelectrophoresis against fresh guinea pig serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of mouse serum or plasma. |
| Form/Appearance | Vial with 1 ml lyophilized antiserum. |
| Concentration | Precipitin titre not less than 1:32 when tested against normal guinea pig plasma in agar-block immunodiffusion titration. |
| Preservatives | No preservative added. |
| Storage | The lyophilised antiserum is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. Reconstitute the lyophilised antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4°C, not refrozen, and preferably used the same day. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other guinea pig serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross- reactivity of this antiserum has not been tested in detail. IE: In immunoelectrophoresis use 2 μl guinea pig plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 ulantiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single radial immunodiffusion use 1 percent antiserum in the gel. |
| 产品资料 |