| 产品名称 | C3C antibody (FITC) | anti C3C antibody (FITC) | Biorbyt |
| 产品货号 | orb21948 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | C3C antibody (FITC) |
|---|---|
| Catalog Number | orb21948 |
| Species/Host | Goat |
| Reactivity | Canine |
| Conjugation | FITC |
| Tested applications | DOT, IEP |
| Target | C3C (FITC). In immunoelectrophoresis against fresh dog serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of dog serum or plasma. |
| Form/Appearance | Vial with 1 ml lyophilized immunoconjugate. |
| Concentration | IgG protein concentration 1 mg/ml. Fluorescein/IgG protein molar ratio (F/P) approximately 1.7. No foreign proteins added. |
| Preservatives | No preservative added, as it may interfere with the antibody activity. |
| Storage | The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate. |
| Note | For research use only. |
| Clonality | Polyclonal |
| Adsorption | Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. |
| Application Notes | The antiserum does not cross-react with any other component of dog plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Conjugation Notes: This antiserum should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro research purposes only. Fluorescein isothiocyanate isomer 1 (FITC). Excitation: 492 nm, emission: 515 nm. Experiment Notes: The IgG (7S) fraction is isolated and purified from the antiserum and contains the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis and double radial immunodiffusion. |
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