| 产品名称 | HeLa Whole Cell Lysate | Biorbyt |
| 产品货号 | orb348668 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | HeLa Whole Cell Lysate |
|---|---|
| Catalog Number | orb348668 |
| Reactivity | Human |
| Tested applications | WB |
| Hazard Information | Non-Toxic |
| Target | HeLa Whole Cell Lysate |
| Form/Appearance | Liquid: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) 10% (v/v) Glycerol |
| Concentration | 1.0 mg/ml |
| Storage | Store vial at -70°C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles. |
| Note | For research use only. |
| Application Notes | ready-to-use HeLa whole cell lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95°C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating. The recommended loading volume per lane is 10-20 μl depending on the size format of your gel. |
| 产品资料 |