产品名称 Human ME2 His tag protein | Biorbyt
产品货号 orb223929
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Human ME2 His tag protein
Catalog Number orb223929
Species/Host Human
Conjugation Unconjugated
Tested applications SDS-PAGE
Sequence MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RN
Target Human ME2 His tag
Form/Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to thebottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to aconcentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots andstored at< 20 °C. Further dilutions should be made in appropriate buffered solutions.
Purity > 95% by SDS-PAGE and HPLC analyses.
MW 64.4 kDa
Source E. coli
Activity Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 iO 0.6 mM.
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