产品名称 Bovine Serum Albumin Fr.V | Biorbyt
产品货号 orb80190
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Bovine Serum Albumin Fr.V
Catalog Number orb80190
Conjugation FITC
Tested applications SDS-PAGE
Target Bovine Serum Albumin Fr.V
Form/Appearance Vial with 1ml lyophilized conjugate.
Concentration BSA concentration 1 mg/ml. Fluorochrome/BSA molar ratio (F/P) 1.2.
Preservatives No preservative added.
Storage The lyophilised conjugate is shipped at ambient temperature and may be stored at 4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
Note For research use only.
Purity Purification according to the method of Cohn (J. Amer. Chem. Soc. 68 (1946), 459). Not less than 90% as tested in immuno electrophoresis. Tested in a concentration of 20 mg/ml at the level of sensitivity of the applied immuno- precipitating techniques, th
Application Notes Conjugation Notes: A proprietary technique for the binding to FITC is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life. Fluorescein isothiocyanate isomer 1. Excitation: 492 nm, emission: 515 nm.

Experiment Notes: Normal serum protein reference sera are shipped lyophilized at ambient temperature. After arrival store at +4°C. P rolonged storage may be at -20°C . After opening the vial, the lyophi lized content is reconstituted by adding 1 ml of sterile distilled water, mixed gently by inversion until complete dissolution is obtained. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted serum may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the reference serum.
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