| 产品名称 | Human Fibronectin protein | Biorbyt |
| 产品货号 | orb81121 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Human Fibronectin protein |
|---|---|
| Catalog Number | orb81121 |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | SDS-PAGE |
| Target | Human Fibronectin |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. The Fibronectin was lyophilized from a concentrated 1mg/ml solution containing 0.05M Tris Cl pH-7.5 and 0.1M NaCl. |
| Storage | Store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. |
| Note | For research use only. |
| Purity | >95.0% as determined by SDS-PAGE. |
| Source | Human Plasma |
| Application Notes | Experiment Notes: Fibronectin is useful for the induction of cell attachment to variety of surfaces including plastic and glass tissue culture labware, petri dishes, coverslips, microcarrier beads, etc. Fibronectin is useful for growth and maintenance of cells in low serum conditions. In general, the reconstituted fibronectin should be diluted with sterile physiological saline or serum-free medium to concentration of 10-50 μg/ml. The plates are treated with the solution, at room temperature 1-2 hours, the supernatant removed and the plates rinsed with fresh media or buffer. Growth media is then added and the coated plates seeded with suspension of freshly harvested cells. Care should be taken to inactivate serum proteases used in the preparation of the cells, since fibronectin is very sensitive to proteolysis. Optimum concentration of fibronectin depends on the types of cell and the particular surface used. Generally, use 1-5 μg/cm2 of growth surface. |
| Solubility (25°C) | Purified fibronectin has a tendency to form insoluble aggregates upon reconstitution. We suggest reconstituting the 1mg Fibronectin with a chaotropic agent such as urea at room temperature at a concentration of 0.2mg/ml using sterile water. Let stand 1-2 |
| 产品资料 |