| 产品名称 | Chicken GH Antagonist protein | Biorbyt |
| 产品货号 | orb80367 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Chicken GH Antagonist protein |
|---|---|
| Catalog Number | orb80367 |
| Reactivity | Gallus |
| Conjugation | Unconjugated |
| Tested applications | Functional Assay, HPLC, SDS-PAGE |
| Target | Chicken GH Antagonist |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1mg/ml) solution with 0.3% NaHCO3 pH-8. |
| Storage | Store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. |
| Note | For research use only. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Phe-Pro-Ala. |
| Purity | >98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Source | E coli |
| Activity | Recombinant Chicken Growth Hormone G119R mutant did not bind to ovine GHR-ECD and was devoid of any biological activity in FDC-P1 3B9 cells. However, in binding experiments that were carried out using chicken liver membranes, both ovine GH and chicken GH showed similar IC50 values in competition with 125I-ovine GH, while the IC50 of G119R mutein was 10-fold higher. These results emphasize the importance of species specificity and indicate the possibility of antagonistic activity of chGH G119R in homologous system. |
| Application Notes | Experiment Notes: Protein quantitation was carried out by: UV spectroscopy at 280 nm using the absorbency value of 0.8 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Growth-Hormone Chicken antagonist in 0.4% NaHCO3 or water adjusted to pH 8-9, not less than 100μg/ml, which can then be further diluted to other aqueous solutions, preferably in a presence of a carrier |
| 产品资料 |