| 产品名称 | Bovine Leptin protein | Biorbyt |
| 产品货号 | orb80494 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Bovine Leptin protein |
|---|---|
| Catalog Number | orb80494 |
| Reactivity | Bovine |
| Conjugation | Unconjugated |
| Tested applications | Functional Assay, HPLC, SDS-PAGE |
| Target | Bovine Leptin |
| Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. |
| Storage | Store at 4°C.for up to two weeks. For long term storage, aliquot and store at -20°C. |
| Note | For research use only. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg. |
| Purity | >95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. |
| Source | E coli |
| Activity | Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. |
| Application Notes | Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.19 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Bovine as Reference Standard. |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Leptin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
| 产品资料 |