产品名称 | WNT-1 antibody | anti WNT-1 antibody | Biorbyt |
产品货号 | orb303962 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | WNT-1 antibody |
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Catalog Number | orb303962 |
Species/Host | Rabbit |
Reactivity | Human |
Conjugation | Unconjugated |
Tested applications | IHC-P, S-ELISA, WB |
Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-1 |
Target | WNT-1 |
Form/Appearance | A sterile filtered antibody solution was lyophilized from PBS, pH 7.2. |
Concentration | 0.1-1.0 mg/ml |
Storage | Store at 4°C for short term and at-20°C for long term storage. |
Note | For research use only. |
Isotype | IgG |
Clonality | Polyclonal |
Purity | Purified by affinity chromatography |
Uniprot ID | P04628 (Human) |
Dilution Range | WB: 0.1-0.2 μg/ml, IHC-P: 62.5 ng/ml |
Application Notes | WB Notes: To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. ELISA Notes: To detect hWNT-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. |
产品资料 |