产品名称 WNT-1 antibody | anti WNT-1 antibody | Biorbyt
产品货号 orb303962
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name WNT-1 antibody
Catalog Number orb303962
Species/Host Rabbit
Reactivity Human
Conjugation Unconjugated
Tested applications IHC-P, S-ELISA, WB
Immunogen Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-1
Target WNT-1
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Purified by affinity chromatography
Uniprot ID P04628 (Human)
Dilution Range WB: 0.1-0.2 μg/ml, IHC-P: 62.5 ng/ml
Application Notes WB Notes: To detect hWNT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Notes: To detect hWNT-1 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 62.5 ng/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
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