产品名称 Collagen IV antibody | anti Collagen IV antibody | Biorbyt
产品货号 orb98273
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Collagen IV antibody
Catalog Number orb98273
Species/Host Rabbit
Reactivity Bovine, Human, Porcine, Rat
Conjugation Unconjugated
Tested applications IHC-Fr, IHC-P, WB
Target Collagen IV
Alternative Names
Form/Appearance Supplied in PBS containing 0.09% sodium azide.
Storage Store at 4°C.or in small aliquots for prolonged storage at-20°C.
Note For research use only.
Clonality Polyclonal
Purity Affinity purified
Clone ID Polyclonal
Application Notes Polyclonal type IV is useful for immunoblotting, immunocytochemistry on fixed cells (methanol fixation) and immunohistochemistry on frozen tissues (fixed in methanol, aceton or 1% paraformaldehyde) when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. For immunohistochemistry on paraffin tissue the sections should be pretreated with 1 mg/ml pepsin in 0.01 N HCl for 30 minutes at room tempeRature. Optimal antibody dilution should be determined by titRation; 1:25-1:100 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100-1:1000 for immunoblotting appliCations.
产品资料