产品名称 | TGF beta 1 antibody | anti TGF beta 1 antibody | Biorbyt |
产品货号 | orb303916 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | |
产品品牌 | Biorbyt |
产品概述 | |
产品详情 | |
Product Name | TGF beta 1 antibody |
---|---|
Catalog Number | orb303916 |
Species/Host | Mouse |
Reactivity | Human |
Conjugation | Unconjugated |
Tested applications | IHC-P, S-ELISA, WB |
Immunogen | Produced in mice using highly pure recombinant human TGF- beta1 as the immunizing antigen |
Target | TGF beta 1 |
Form/Appearance | A sterile filtered antibody solution was lyophilized from PBS. |
Concentration | 0.1-1.0 mg/ml |
Storage | Store at 4°C for short term and at-20°C for long term storage. |
Note | For research use only. |
Isotype | IgG |
Clonality | Monoclonal |
Purity | Protein G affinity chromatography. |
Uniprot ID | P01137 (Human) |
Dilution Range | WB: 2.0-4.0 μg/ml, IHC-P: 5.0 μg/ml |
Application Notes | WB Notes: To detect Human TGF-β1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-β1 is 2.0-4.0 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-β2 and TGF-β3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution. ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant Human TGF-β1 when used in conjunction with compatible secondary reagents. IHC Notes: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. |
产品资料 |