产品名称 TGF beta 1 antibody | anti TGF beta 1 antibody | Biorbyt
产品货号 orb303916
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name TGF beta 1 antibody
Catalog Number orb303916
Species/Host Mouse
Reactivity Human
Conjugation Unconjugated
Tested applications IHC-P, S-ELISA, WB
Immunogen Produced in mice using highly pure recombinant human TGF- beta1 as the immunizing antigen
Target TGF beta 1
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
Clonality Monoclonal
Purity Protein G affinity chromatography.
Uniprot ID P01137 (Human)
Dilution Range WB: 2.0-4.0 μg/ml, IHC-P: 5.0 μg/ml
Application Notes WB Notes: To detect Human TGF-β1 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-β1 is 2.0-4.0 ng/lane, under non-reducing conditions.

In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-β2 and TGF-β3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.
ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 100 pg/ml of recombinant Human TGF-β1 when used in conjunction with compatible secondary reagents.
IHC Notes: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5.0 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
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