| 产品名称 | TNF-alpha antibody | anti TNF-alpha antibody | Biorbyt |
| 产品货号 | orb303931 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | TNF-alpha antibody |
|---|---|
| Catalog Number | orb303931 |
| Species/Host | Mouse |
| Reactivity | Human |
| Conjugation | Unconjugated |
| Tested applications | IHC-P, S-ELISA, WB |
| Immunogen | Produced in BALB/c mice using highly pure recombinant human TNF-alpha as the immunizing antigen |
| Target | TNF-alpha |
| Form/Appearance | A sterile filtered antibody solution was lyophilized from PBS. |
| Concentration | 0.1-1.0 mg/ml |
| Storage | Store at 4°C for short term and at-20°C for long term storage. |
| Note | For research use only. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Purity | Protein G affinity chromatography. |
| Uniprot ID | P01375 (Human) |
| Dilution Range | WB: 0.5-1.0 μg/ml, IHC-P: 15.0-20.0 μg/ml |
| Application Notes | WB Notes: To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human TNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human TNF-α when used with Biorbyt' biotinylated antigen affinity purified anti-Human TNF-α IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 15.0-20.0 μg/ml overnight at 4°C. An HRP-labeled polymer detection system was used with DAB Chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. |
| 产品资料 |