产品名称 sTRAIL antibody | anti sTRAIL antibody | Biorbyt
产品货号 orb303942
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name sTRAIL antibody
Catalog Number orb303942
Species/Host Mouse
Reactivity Human
Conjugation Unconjugated
Tested applications IHC-P, S-ELISA, WB
Immunogen Produced in BALB/c x ICR F1 mice using highly pure (>98%) recombinant human TRAIL/APO2L as the immunizing antigen
Target sTRAIL
Alternative Names
Form/Appearance A sterile filtered antibody solution was lyophilized from PBS.
Concentration 0.1-1.0 mg/ml
Storage Store at 4°C for short term and at-20°C for long term storage.
Note For research use only.
Isotype IgG
Clonality Monoclonal
Purity Protein A affinity chromatography.
Uniprot ID P50591 (Human)
Dilution Range WB: 0.25-0.50 μg/ml, IHC-P: 2.5 μg/ml-1.5 μg/ml
Application Notes WB Notes: To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.
ELISA Notes: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human sTRAIL/APO2L when used with Biorbyt' biotinylated antigen affinity purified anti-human sTRAIL/APO2L
IHC Notes: This antibody stained formalin-fixed, paraffin-embedded sections of human renal cell carcinoma.  The recommended concentration is 2.5 µg/mL- 1.5 µg/mL with an overnight incubation at 4°C.  An HRP-labeled polymer detection system was used with a DAB chromogen.  Heat induced antigen retrieval with a pH 9.0 buffer is recommended.  Optimal concentrations and conditions may vary.
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