| 产品名称 |
Human Noggin protein |
| 产品货号 |
orb80092 |
| 产品价格 |
现货询价，电话：010-67529703
|
| 产品规格 |
|
| 产品品牌 |
Biorbyt |
| 产品概述 |
|
| 产品详情 |
|
| Product Name |
Human Noggin protein |
| Catalog Number |
orb80092 |
| Conjugation |
Unconjugated |
| Tested applications |
Functional Assay, HPLC, SDS-PAGE |
| Target |
Noggin |
| Alternative Names |
(click to expand)
Nog Protein, NOGG_HUMAN Protein, Noggin Protein
|
| Form/Appearance |
Lyophilized from 0.2 um filtered solution in 30% CH3CN, 0.1% TFA. |
| Storage |
Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Note |
For research use only. |
| Protein Sequence |
MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGQRCGW IPIQYPIISE CKCSC. |
| Purity |
> 95.0% as determined by SDS-PAGE. |
| Biological Origin |
E.coli |
| Activity |
The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 ug/ml of Noggin. |
| Application Notes |
Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.76 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Noggin as Reference Standard. |
| Solubility (25°C) |
It is recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions. |
| 产品资料 |
|