产品名称 Human Stem Cell Factor protein | Biorbyt
产品货号 orb80102
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Human Stem Cell Factor protein
Catalog Number orb80102
Conjugation Unconjugated
Tested applications Functional Assay, HPLC, SDS-PAGE
Target Human Stem Cell Factor
Alternative Names
Form/Appearance Lyophilized from concentrated (1mg/ml) solution in water containing 10mM Acetic acid.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Protein Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.
Purity > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Biological Origin E.coli
Activity The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is 2 ng/ml, corresponding to Specific Activity of 500,000 IU/mg.
Application Notes Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using standard solution of Stem Cell Factor as Reference Standard.
Solubility (25°C) It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
产品资料