产品名称 Rat IL10 protein | Biorbyt
产品货号 orb80069
产品价格 现货询价,电话:010-67529703
产品规格
产品品牌 Biorbyt
产品概述
产品详情
Product Name Rat IL10 protein
Catalog Number orb80069
Conjugation Unconjugated
Tested applications Functional Assay, HPLC, SDS-PAGE
Target rIL 10
Alternative Names
Form/Appearance The protein was lyophilized containing 25mM sodium phosphate buffer pH-7.25 and 100mM NaCl.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Protein Sequence The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Lys-Gly-His.
Purity > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
Biological Origin E.coli
Activity The ED50 as determined by the dose dependent inhibition of MC/9 proliferation is less than 10 ng/ml.
Application Notes Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.62 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-10 as Reference Standard.
Solubility (25°C) It is recommended to reconstitute the lyophilized IL-10 Rat Recombinant in sterile H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
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