| 产品名称 | Vimentin antibody | anti Vimentin antibody | Biorbyt |
| 产品货号 | orb131684 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | |
| 产品品牌 | Biorbyt |
| 产品概述 | |
| 产品详情 | |
| Product Name | Vimentin antibody |
|---|---|
| Catalog Number | orb131684 |
| Species/Host | Gallus |
| Conjugation | Unconjugated |
| Target | Vimentin |
| Alternative Names | (click to expand) |
| Form/Appearance | Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative. |
| Concentration | 300 μg/ml (based on absorbance at 280 nm) |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Note | For research use only. |
| Isotype | IgY |
| Clonality | Polyclonal |
| Purity | Affinity-Purified |
| Dilution Range | IHC-P: 1:1000-2000, WB: 1:2000-5000 |
| Application Notes | Each of the three antibodies were analyzed by immunohistochemistry (at a dilutionof 1:2000) using fluorescein-labeled goat anti-chicken IgY as the secondary reagent. Experiment Notes: Chicken were immunized with three synthetic peptide / keyhole limpet hemocyanin(KLH) conjugates. These synthetic peptides corresponded to different regions of thevimenin gene product, but are shared between the Human (NP_003371, NCBI) andmouse (NP_035831, NCBI) protein sequences. After repeated injections, immuneeggs were collected, and the IgY fractions were Purified from the yolks. These IgYfractions were then affinity-Purified using a peptide column, and the concentrationsof the eluates adjusted to 300 ug/ml. Finally, equal volumes of each of the threeaffinity-Purified anti-peptide antibodies were mixed, and the preparation was filtersterilized. |
| 产品资料 |