Recombinant HEK293 cell line expressing human TMEM16B (transmembrane protein 16B, also called as anoctamin 2, calcium-activated chloride channel (ANO2), accession number NM_001278596).

19475416

TMEM16B, transmembrane protein 16B, anoctamin 2, calcium-activated chloride channel, ANO2

Each vial contains ~1.5 X 10⁶ cells in 1 ml of 10% DMSO.

Human TMEM16B channel has been stably expressed in HEK293 cell line and its expression was confirmed by Western blotting. The CaCC activity of TMEM16B was characterized by an assay based on a halidesensitive yellow fluorescent protein (YFP) mutant whose fluorescence is quenched by increasing halide concentration. When TMEM16B-expressed HEK293 cells were stimulated with ionomycin to raise the intracellular level of Ca²⁺, TMEM16B produced I- influx in HEK293, triggering a rapid decrease of fluorescence by the transfected YFP mutant. The ionomycin-induced I- influx through TMEM16B was blocked by niflumic acid, a CaCC channel blocker.

HEK293 cells

The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species.

Thaw Medium 1 (BPS Cat. #60187): MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (Invitrogen #26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01)

Growth Medium 1F (BPS Cat. #79540): Thaw Medium 1 (BPS Cat. #60187) plus 100 µg/ml of Hygromycin B (Hyclone #SV30070.01) to ensure the recombinant expression is maintained. It may be necessary to adjust the percentage of CO2 in the incubator, depending on the NaHCO3 level in the basal medium. TMEM16B-HEK293 cells should exhibit a typical cell division time of approximately 24 hours.

See data sheet for detailed culturing and assay protocol.

Store in liquid nitrogen immediately upon receipt.

• Drug compound screening for activators or inhibitors of TMEM16B
• Functional assays for TMEM16B
• Efficient antigen for mouse immunization

1. Verkman A.S. and Galietta L.J.V. (2009) Cholide channels as drug targets. Nature Reviews 8:153-171.

2. Scudieri P. et al. (2012) The anoctamin family: TMEM16A and TMEM16B as calciumactivated chloride channels. Exp. Physiol. 97(2):177-183.

3. Stöhr H. et al. (2009) TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals. J. Neurosci. 29(21):6809-6818.

License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Avoid freeze/thaw cycles

Ion Channel

Cell Line