| 产品名称 | ICOS/NFAT Reporter-Jurkat Recombinant Cell Line - Immunotherapy Cell Line Rentals - Cell Line Rentals |
| 产品货号 | 79668 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: ICOS is a costimulatory molecule of the CD28 cell surface receptor superfamily that is expressed on activated T-cells. ICOS is involved in T-cell responses upon binding with its ligand, ICOSL (also known as B7-H2, CD275), which is normally expressed on B-cells, dendritic cells and monocytes. ICOS expression confers an activated phenotype and a strong suppressive capacity to intra-tumoral regulatory T-cells. The ICOS/ICOSL pathway is a key target for cancer immunotherapy.
Description: Recombinant Jurkat T cell expressing firefly luciferase gene under the control of NFAT response elements with constitutive expression of a chimeric receptor consisting human ICOS (also known as inducible T-cell costimulator or CD278, Genbank Accession #NM_012092) and the cytoplasmic domain of human CD3 zeta.
Materials Required But Not Provided: • Assay Medium: Thaw Medium 2 (BPS Bioscience #60184) • ICOSL-CHO K1 Recombinant Cell Line (BPS Bioscience #79635) and its recommended growth medium (Growth Medium 3D, BPS Bioscience #79539) • 96-well tissue culture-treated white clear-bottom assay plate • One-Step luciferase assay system (BPS Bioscience #60690) for measuring firefly luciferase activity • Luminometer Synonym(s): T-cell costimulator, CD278
Host Cell line: Jurkat T Cell
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination.
Growth Media: Thaw Medium 2 (BPS Bioscience #60184): RPMI1640 medium (Life Technologies #A10491-01) supplemented with 10% FBS (Life Technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01). Growth Medium 2F (BPS Bioscience #79669): Thaw Medium 2 (BPS Bioscience #60184), 1 mg/ml of Geneticin (Life Technologies #11811031), and 0.5 μg/ml of Puromycin (Takara, #631306). Format: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO in FBS. Instructions for use: See data sheet for detailed culturing and assay protocol.
Storage / Stability: Store in liquid nitrogen immediately upon receipt.
Application(s): • Screen for agonists of ICOS signaling in a physiologically relevant cellular context • Characterize T cell-mediated immune responses of ICOS and its interaction with ICOSL
Notes: LICENSE DISCLOSURE: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
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