The CBX1 Inhibitor Screening Assay Kit is designed to measure the inhibition of CBX1 binding to its substrate. The kit comes in a convenient AlphaLISA® format, with enough biotinylated peptide substrate, assay buffer, detection buffer and purified GST-tagged CBX1 chromodomain to perform a total of 384 enzyme reactions. The key to the kit is the specific binding of the CBX1 chromodomain to the methylated-peptide substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing CBX1 and an inhibitor of choice is incubated with the biotinylated substrate for one hour. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.

cbx, cbx1, activity assay kit, AlphaLisa, AlphaScreen, Alpha Screen

Green and blue dyes that absorb light in the AlphaScreen signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen assays.

See data sheet for detailed protocol.

Stable at least 12 months from date of receipt, when stored as directed

Useful for the study of chromodomain binding assays, screening inhibitors, and selectivity profiling.

Kaustov, L., et al. J. Biol. Chem. 2011; 286(1):521-9.

AlphaScreen^® and AlphaLISA^® are registered trademarks of PerkinElmer, Inc.

Avoid freeze/thaw cycles

Methyl-lysine Reader

Assay Kit