| 产品名称 | LIGHT-CHO Recombinant Cell Line - Cell Lines |
| 产品货号 | 79262 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 Vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: LIGHT (TNFSF14) is a key component of the communication system that controls the responses of T-Cells. LIGHT activates two cell surface receptors, the Herpesvirus Entry Mediator (HVEM) and the Lymphotoxin-beta Receptor (LTβR). The LIGHT-HVEM axis is an important costimulatory pathway for T cells, it has been shown to activate NF-κB; LIGHT/HVEM pair are co-stimulatory immune checkpoint molecules for cancer immunotherapy, LIGHT expressing cells have been shown to be more potent than the soluble LIGHT for activating HVEM expressed on T cell surface.
Description: Recombinant stable clonal CHO cell line constitutively expressing full length human LIGHT protein, also known as TNFSF14, (Genbank #NM_003807). Surface expression is confirmed by flow cytometry.
Synonym(s): TNFSF14, light cho
Supplied As: Each vial contains ~ 2 x 10^6 cells in 1 ml of 10% DMSO in FBS.
Host Cell line: CHO K1, Chinese Hamster Ovary
Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza, #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Lonza, #LT07-518) was used as a positive control.
Growth Media: Thaw Medium 3 (BPS Bioscience, #60186): Ham’s F-12 medium (Hyclone #SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone #SV30010.01). Growth Medium 3D (BPS Bioscience, #79539): Thaw Medium 3 (BPS Bioscience, #60186) plus 1 mg/ml G418 (Thermo Fisher, #11811031). Instructions for use: See data sheet for detailed culturing and assay protocol.
Storage / Stability: Store in liquid nitrogen immediately upon receipt.
Application(s): This cell line is useful for LIGHT binding assays, flow cytometry, or for screening for LIGHT antibodies. Also useful for immune checkpoint co-stimulatory assay when co-cultured with HVEM/NF-κB-luciferase/Jurkat reporter cell line (BPS Bioscience).
Reference(s): 1. Chen, et.al. (2013) Molecular mechanisms of T cell co-stimulation and co-inhibition. Nat Rev Immunol. 2013 April ; 13(4): 227–242. 2. Steinberg, et.al. (2014) The Signaling Networks of the Herpesvirus Entry Mediator (TNFRSF14) in Immune Regulation. Immunol Rev. 2011 November; 244(1): 169–187. 3. Rio, et.al. (2014) Therapeutic blockade of LIGHT interaction with HVEM and LTβR attenuates in vivo cytotoxic allogeneic responses. Transplantation. 2014 December 15; 98(11): 1165–1174. Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Immunotherapy
Product Type: Cell Line
Data shown is lot-specific. Contact us for specific information on other lots.
|
| 产品资料 |
