| 产品名称 | HVEM/TCR Activator - CHO Recombinant Cell Line - Cell Lines |
| 产品货号 | 79551 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 2 vials |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Background: HVEM is a member of the TNFR superfamily that is expressed on T cells, B cells, NK cells and antigen-presenting cells. HVEM is a bidirectional switch that regulates T-cell activation in either a costimulatory or coinhibitory fashion, depending on the binding partner. HVEM can act as both ligand and receptor; the binding of HVEM to BTLA (IgSF) or CD160 on Effector T cells delivers a coinhibitory signal; alternately, the binding of HVEM to either of two tumor necrosis factor ligands, LIGHT or lymphotoxin-α, delivers a costimulatory signal. The HVEM/BTLA axis are co-inhibitory immune checkpoint molecules that play important roles in the blockade of T cell-mediated immune responses. The binding of BTLA, a receptor expressed on activated T-cells, to its ligand HVEM, found on melanoma and other cancers, negatively regulates T cell immune responses and allows cancer cells to escape immune surveillance. This co-inhibitory signal of the HVEM/BTLA interaction can be reversed by treatment with a BTLA blocking antibody. The HVEM/BTLA/LIGHT pathway is also involved in regulating autoimmune responses, making these proteins promising therapeutic targets for a number of diseases.
Description: Recombinant CHO-K1 cells constitutively expressing human HVEM (Herpes Virus Entry Mediator), also known as CD270 or TNFRSF14 (Tumor Necrosis Factor Receptor Superfamily Member 14), GenBank Accession No. NM_003820, and an engineered T cell receptor (TCR) activator. Materials Required But Not Provided: • BTLA/Jurkat_NFAT reporter cell line (BPS Bioscience #79476) • Assay medium: Thaw Medium 2 (BPS Bioscience #60184) • Growth Medium 3A (BPS Bioscience #60188) • Thaw Medium 3 (BPS Bioscience #60186) Anti-BTLA neutralizing antibody (for testing, n/a) • Anti-HVEM neutralizing antibody (for testing, n/a) • 96-well tissue culture-treated white wall clear-bottom assay plate • One-Step luciferase assay system (BPS Bioscience #60690) • Luminometer Formulation: Each vial contains 2 x 106 cells in 1 ml of 10% DMSO
Host Cell line: CHO-K1
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor®GeM Mycoplasma Detection kit (Sigma-Aldrich, #MP0025) to confirm the absence of Mycoplasma species. Growth Media: Thaw Medium 3 (BPS Bioscience #60186): Ham’s F-12 medium (Hyclone # SH30526.01) supplemented with 10% FBS (Life technologies #26140-079), 1% Penicillin/Streptomycin (Hyclone SV30010.01). Growth Medium 3A (BPS Bioscience #60188): Thaw Medium 3 (BPS Bioscience #60186) plus 1 mg/ml of Geneticin (Life Technologies #11811031) and 500 μg/ml of Hygromycin B (Hyclone #SV30070.01) to ensure recombinant expression. Instructions for use: See data sheet for detailed culturing and assay protocol
Storage / Stability: Immediately upon receipt, store in liquid nitrogen.
Application(s): •Screen for activators or inhibitors of BTLA/HVEM interaction in cell-based co-inhibitory bioassay.
•Characterize the biological activity of HVEM and its interactions with its receptor BTLA. Reference(s): 1. BTLA marks a less cytotoxic T-cell subset in diffuse large B-cell lymphoma with high expression of checkpoints. Quan L. et al. Exp Hematol. 2018 Apr. 60:47-56 2. Newly Emerging Immune Checkpoints: Promises for Future Cancer Therapy. Torphy RJ, et al. Int J Mol Sci. 2017 Dec 6;18(12): E2642. Review. 3. Design of short peptides to block BTLA/HVEM interactions for promoting anticancer T-cell responses. Spodzieja M, et al. PLoS One. 2017 Jun 8;12(6): e0179201 4. BTLA marks a less-differentiated tumor-infiltrating lymphocyte subset in melanoma with enhanced survival properties Cara L Haymaker et al. OncoImmunology August 2015, 4(8):e1014246 5. Immunotherapy: Beyond Anti-PD-1 and Anti-PD-L1 Therapies. Antonia SJ et al. Am Soc Clin Oncol Educ Book. 2016; 35:e450-8 6. Knockdown of HVEM, a Lymphocyte Regulator Gene, in Ovarian Cancer Cells Increases Sensitivity to Activated T Cells. Zhang T, et al. Oncol Res. 2016;24(3):189-96 7. Increased BTLA and HVEM in gastric cancer are associated with progression and poor prognosis. Lan X, et al. Onco Targets Ther. 2017 Feb 16; 10: 919-926. 8. BTLA identifies dysfunctional PD-1-expressing CD4+ T cells in human hepatocellular carcinoma. Zhao Q, et al. Oncoimmunology. 2016 Nov 8, 5 (12): e1254855. 9. Loss of the HVEM Tumor Suppressor in Lymphoma and Restoration by Modified CAR-T Cells. Boice M, et al. Cell. 2016 Oct 6;167(2):405-418 10. HVEM is a TNF Receptor with Multiple Regulatory Roles in the Mucosal Immune System Jr-Wen Shui and Mitchell Kronenberg. Immune Network April, 2014, 14(2): 67-72. 11. The Signaling Networks of the HVEM/BTLA in Immune Regulation Marcos Steinberg, et al. Immunol Rev. 2011 November; 244(1): 169–187 Notes: License Disclosure: Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit the use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.
Scientific Category: Immunotherapy
Product Type: Cell line
Data shown is lot-specific. Contact us for specific information on other lots.
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