Human Programmed Cell Death 1 Ligand 1 (PD-L1), also known as CD274 and B7 homolog 1 (B7-H1), PDCD1 ligand 1, programmed death ligand 1, PDCD1L1, GenBank Accession No. NM_014143, a.a. 19-239, with a C-terminal FLAG-tag, expressed in a HEK293 cell expression system. MW = 26 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Q9NZQ7

Programmed Cell Death 1 Ligand 1,CD274 and B7 homolog 1 (B7-H1), PDCD1 ligand 1, programmed death ligand 1, PDCD1L1

≥90%

10 nM PD-1, Biotinlabeled (BPS Cat. #71109) was mixed with varying amounts of PD-L1, FLAG-tag (BPS Cat. #71183) in PD-1 Assay Buffer for 1 hr. at RT. FLAG-acceptor beads were added and incubated for 30 min at RT followed by strep-conjugated donor beads for 30 min at RT. Alpha counts were read.

8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.

Aqueous buffer solution.

At least 6 months at –80°C.

Useful for studying protein binding and for screening small molecules and antibodies.

1. Freeman, G.J., et al. J. Exp. Med. 2000; 192(7): 1027-1034

2. Dong, H., et al., Nat. Med. 1999; 5(12): 1365-1369.

HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration.

Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.

Immunotherapy

Recombinant Protein