Recombinant HEK293 cell constitutively expressing full length human GITR. Surface expression is confirmed by flow cytometry.

CHO-K1

This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Lonza #LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat # LT07-518) was used as a positive control.

Thaw Medium 1 (BPS, #60187): MEM medium (Hyclone, #SH30024.01) supplemented with 10% FBS (Invitrogen, #26140-079), 1% non-essential amino acids (Hyclone, #SH30238.01), 1 mM Na pyruvate (Hyclone, #SH30239.01), 1% Penicillin/Streptomycin (Hyclone, #SV30010.01).

Growth Medium 1F (BPS, #79540): Thaw Medium 1 (BPS, #60187) plus 100 µg/ml Hygromycin B (Thermo Fisher, #10687010).

Store in liquid nitrogen immediately upon receipt.

This cell line is a good candidate for binding analyses and for studying GITR receptor activity.

License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suitability of the cell line for any particular use, and does not accept any liability in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bpsbioscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.

Immunotherapy

Cell Line