R-Phycoerythrin-labeled anti-PD-L1 recombinant murine/human antibody recognizing the PD-1 binding region of human PD-L1. This antibody does not cross-react with mouse PD-L1, but does cross-react with monkey (M. fascicularis) PD-L1. It has not been tested with other species.

For flow cytometry, we recommend incubating cells on ice for 30 minutes with 1-20 µg/ml of anti-human PD-L1 antibody, PE-labeled.

8 mM Na phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.

Protein A affinity chromatography from HEK293 supernatants.

Useful for labeling cells expressing PD-L1 for flow cytometry, immunofluorescence microscopy, and live cell imaging.

1. Freeman, G.J., et al., J. Exp. Med. 2000; 192(7): 1027-1034.

2. Dong. H., et al., Nat. Med. 1999; 5(12):1365-1369.

Avoid freeze/thaw cycles. Store diluted antibody with carrier protein (BSA 0.1 – 0.5%) at 4°C, stable for 2 weeks.

Immunotherapy

Antibody