The mouse CTLA4:B7-1 TR-FRET Assay is designed to measure the inhibition of mouse CTLA4 binding to mouse B7-1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated mCTLA4, mB7-1 protein, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.

Fluorescence microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET)

Adjustable micropipettor and sterile tips

CD152, cluster of differentiation 152, B7, HTRF

See assay kit data sheet for detailed protocol

At least 6 months from date of receipt when stored as directed.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

Huxley, P., et al. Chem Biol. 2004 Dec;11(12):1651-8.

Avoid freeze/thaw cycles

Immunotherapy

Assay kit