| 产品名称 | Mouse PKM2, His-tag |
| 产品货号 | 50296 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Mouse
Host Species: E. coli
MW: 58 kDa
Genbank #: NM_011099
Tag(s): N-terminal His-tag
a.a: 2–end
Background: PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
Description: Murine Pyruvate kinase M2 (PKM2), also known as PKM1/M2, isoform a (GenBank Accession No. NM_011099), a.a. 2-end, with N-terminal His-tag, MW = 58 kDa, expressed in an E. coli expression system.
UniProt P52480
Synonym(s): pyruvate kinase muscle, PKM2, cytosolic thyroid hormone-binding protein,CTHBP, thyroid hormone-binding protein 1, THBP1, opa-interacting protein 3,OIP3
Specific Activity: ≥11 pmol/min/µg.
Assay Conditions: A 50 µl PKM2 reaction is conducted in a
buffer containing 50 mM Tris (pH 7.4),
100 mM MgCl2, 250 mM KCl, 40 mM ADP,
100 mM phosphoenolpyruvate (PEP) at
room temperature for 15 min. ATP
production is detected using Kinase-Glo
Luminescent Kinase Assay Platform.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Spoden G.A. et al., Exp. Cell Res. 315 (16), 2765-2774 (2009).
2. Christofk H.R. et al., Nature 452 (7184), 230-233 (2008). Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Metabolic Enzymes-Kinase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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