Human USP8 (Ubiquitin-Specific Protease 8) or UBPY, GenBank Accession No. NM_005154, a.a. 2-1118(end) with an N-terminal FLAG-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.

P40818

USP 8, Ubiquitin-Specific Protease 8, UBPY, USP-8

6.5 pmole/min/µg

Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP8 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.

45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG peptide, 10% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Naviglio, S., et al. (1998). EMBO J. 17(12):3241-50.
2. Niendorf, S., et al. (2007). Mol Cell Biol. 727(13):5029-39.
3. Mizuno, E., et al. (2005). Mol. Biol. Cell. 16(11): 5163-74.

Avoid freeze/thaw cycles

Ubiquitins: Deubiquitinase (DUB)

Recombinant Protein